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Non-specific control IgG, Mouse Monoclonal Antibody
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Catalog Number
M-1249
- Product Name Non-specific control IgG, Mouse Monoclonal Antibody
- Product Description Mouse anti-Non-specific control IgG Monoclonal Antibody (Unconjugated), suitable for WB, IHC, ICC, FC, ELISA.
- Application(s) ELISA, FC, ICC, IHC, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control. N/A
- Species Reactivity Non-Reactive (Negative Control)
- Immunogen Description None. This control IgG has no known binding ability.
- Conjugate Unconjugated
- Purity Description Immunoglobulin purified using Protein G column. Purity analysed using gel electrophoresis.
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Non-specific control IgG Monoclonal Antibody (Unconjugated), suitable for WB, IHC, ICC, FC, ELISA.
- Application(s) ELISA, FC, ICC, IHC, WB
- Application Details Recommended for use as a control for Western Blotting, immunohistochemistry and FACS at a concentration equal to that of the test antibody. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Non-specific control IgG
- Specificity No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control. N/A
- Target Host Species Species Independent
- Species Reactivity Non-Reactive (Negative Control)
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG1, kappa
- Clone Name X63
- Conjugate Unconjugated
- Immunogen Description None. This control IgG has no known binding ability.
- Purity Description Immunoglobulin purified using Protein G column. Purity analysed using gel electrophoresis.
- Format Lyophilized
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions Store lyophilized antibody at 2-8°C. Keep reconstituted antibody at -20°C to -80°C for long-term storage. For short term keep at 2-8°C. We suggest that the customer aliquots the antibody into smaller lots to avoid repeated freezing and thawing.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Scientific Background Commonly used mouse IgG1 negative control antibody clone derived from a Balb/c myeloma and is recommend as a negative control for a variety of immunohistochemical applications where mouse IgG experimental antibodies are use. To date no published reactivity as been associated with X63.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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General References
Rogers ML et al (2006) Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. J Neurosci Methods. Nov 15;158(1):109-20.
Ross AH et al (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. Proc Natl Acad Sci U S A. Nov;81(21):6681-5
Radbruch A., Liesegang B. and Rajewsky K. (1980) Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class Proc Natl Acad Sci U S A. 1980 May;77(5):2909-13.