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TrkB (pS478) Positive Control Cell Lysate (Mouse NSC-34)
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Catalog Number
CL-2107
- Product Name TrkB (pS478) Positive Control Cell Lysate (Mouse NSC-34)
- Product Description This cell lysate is suitable as positive control for Western Blotting, to confirm TrkB phosphorylation at amino acid S478 (rat/mouse) or S479 (human), respectively, using TrkB (pS478/479) rabbit antibody R-1718-50. It is particular useful for complex Western Blotting samples to identify TrkB (pS478/479) immunoreactive bands. This lysate has been prepared by triggering TrkB phosphorylation in retinoic acid-treated mouse NSC34 cells with mature BDNF, and subsequent processing with RIPA buffer. The cell lysate is provided lyophilised for extended stability.
- Alternative Names GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; pTrkB; phosphoTrkB; phospho-TrkB
- Application(s) WB
- Purity Description This lysate has been prepared in RIPA buffer as crude cell lysate.
- Regulatory Status For research use only.
Product Info
- Product Description This cell lysate is suitable as positive control for Western Blotting, to confirm TrkB phosphorylation at amino acid S478 (rat/mouse) or S479 (human), respectively, using TrkB (pS478/479) rabbit antibody R-1718-50. It is particular useful for complex Western Blotting samples to identify TrkB (pS478/479) immunoreactive bands. This lysate has been prepared by triggering TrkB phosphorylation in retinoic acid-treated mouse NSC34 cells with mature BDNF, and subsequent processing with RIPA buffer. The cell lysate is provided lyophilised for extended stability.
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Related Products
Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479), Rabbit Polyclonal Antibody
- Application(s) WB
- Application Details Western Blotting (5 - 10 µg loading per lane). Biosensis recommends optimal loading amounts should be determined by the end user.
- Product Validation This product has been validated for TrkB (S478/479) phosphorylation by Western Blotting, using rabbit antibody R-1718-50 to TrkB (pS478/479). Treatment of blotting membrane with lambda-phosphatase obliterates TrkB pS478/479 immunoreactivity.
- Target Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479)
- Target Host Species Mouse
- Homology This cell lysate is suitable as positive control for human, mouse and rat TrkB (pS478/479).
- Purity Description This lysate has been prepared in RIPA buffer as crude cell lysate.
- Physical State Solid.
- Format Lyophilized from a RIPA cell lysate preparation, without preservatives.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile, ultrapure water to achieve a protein concentration of 1 mg/mL (determined by Bradford Assay). Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
- Storage Instructions Store lyophilized lysate at 2-8°C. After reconstitution divide into single-use aliquots and store at -80°C for long-term storage. Aliquots should be used within 1 hour after thawing. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; pTrkB; phosphoTrkB; phospho-TrkB
- NCBI Organism Mus musculus
- Scientific Background Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia (Ref: uniprot.org).
- Research Area Neurotrophins, neurotrophin receptors.
- Hazardous Statement No components at sufficient quantity are present to cause harm. However, exert standard safe laboratory practices such as wearing gloves and safety glasses when handing buffers that contain SDS.
- Shipping Temperature 25°C (ambient)
- Country of Manufacture Australia
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
- General References Lai K-O. et al. (2012), Nature Neuroscience 15, 1506-1515, doi:10.1038/nn.3237.Zhao L. et al. (2009), Journal of Cell Science 122, 3123-3136, doi: 10.1242/jcs.047712.