Glial fibrillary acidic protein (GFAP), Chicken Polyclonal Antibody

Product Info

Product Name Glial fibrillary acidic protein (GFAP), Chicken Polyclonal Antibody
Product Description google
Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
Alternative Names Astrocyte; Glial fibrillary acidic protein; GFAP;
Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
Antibody Host Chicken
Antibody Type Polyclonal
Specificity The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
Immunogen Description Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Conjugate Unconjugated
Purity Description IgY
Regulatory Status For research use only.

Specifications

Product Description
Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). WB: A dilution of 1:5,000 is recommended. ICC: A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Glial fibrillary acidic protein (GFAP)
Specificity The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Target Host Species Human
Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
Antibody Host Chicken
Antibody Type Polyclonal
Antibody Isotype IgY
Conjugate Unconjugated
Immunogen Description Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Purity Description IgY
Format Lyophilized IgY preparation, with sodium azide.
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Astrocyte; Glial fibrillary acidic protein; GFAP;
Uniprot Number Q28115
Uniprot Number/Name Q28115 (GFAP_BOVIN)
Scientific Background Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
Left: Analysis of GFAP expression (green) in mouse hippocampus section by Immunohistochemistry. Section was stained with chicken anti-GFAP antibody (1:5,000) and co-stained with rabbit antibody to FOX3/NeuN (R-3770-100, 1:5,000, red). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% PFA, mouse brain was post fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The GFAP antibody stains a network of astroglial cells, while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons. Right: Western blot analysis of GFAP expression in whole rat (2) and mouse (3) brain lysates using chicken anti-GFAP antibody (1:5,000). The strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.

Citations & References

Specific References
Britz J et al. (2024) Sex-Dependent Effects of Chronic Circadian Disruption in AβPP/PS1 Mice J Alzheimers Dis. 97(2): 855 Application: Mouse IF

Stallings NR et al. (2023) Long-term normalization of calcineurin activity in model mice rescues Pin1 and attenuates Alzheimer’s phenotypes without blocking peripheral T cell IL-2 response Alzheimers Res Ther. 15(1):179. Application: Mouse, IHC (IF).

Hascup KN et al. (2020) Riluzole attenuates glutamatergic tone and cognitive decline in AβPP/PS1 mice. J Neurochem. [Epub ahead of print] Application: Mouse, IHC(IF).
General References Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.