Ionized Calcium Binding Adapter Molecule 1 (IBA1), Chicken Polyclonal Antibody

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Catalog Number
C-2118

    Product Info

  • Product Name Ionized Calcium Binding Adapter Molecule 1 (IBA1), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Ionized Calcium Binding Adapter Molecule 1 (IBA1), Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC
  • Alternative Names IBA-1; AIF1; AIF-1 G1; Allograft inflammatory factor 1; Ionized calcium binding adapter molecule 1; Protein G1
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human. Species cross-reactivity includes rat and mouse.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description C-terminal peptide of human IBA1. The antibody has been made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin (KLH).
  • Conjugate Unconjugated
  • Purity Description IgY Fraction
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Ionized Calcium Binding Adapter Molecule 1 (IBA1), Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC
  • Related Products Ionized calcium-binding adapter molecule 1 (IBA1), Rabbit Polyclonal Antibody
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Ionized calcium binding adapter molecule 1 (IBA1)
  • Specificity Human. Species cross-reactivity includes rat and mouse.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description C-terminal peptide of human IBA1. The antibody has been made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin (KLH).
  • Purity Description IgY Fraction
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names IBA-1; AIF1; AIF-1 G1; Allograft inflammatory factor 1; Ionized calcium binding adapter molecule 1; Protein G1
  • Uniprot Number P55008
  • Uniprot Number/Name P55008 (AIF1_HUMAN)
  • Scientific Background Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. (Ref: uniprot.org)
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left:Rat spinal cordsection stained with C-2118-50 (red) and nuclear DNA (DAPI stain, blue) by Immunohistochemistry.Microglia are visualized in a confocal Z stack as they are small cells spreading in three dimensions. Right:Western blot analysis of C-2118-50 (green, 1:1000) with a band at approx. 15kDa. Lane 1: protein standard (red); Lane 2: Rat spleen homogenate.

    Citations & References