Tubulin beta chain, Mouse Monoclonal Antibody

Product Info

Product Name Tubulin beta chain, Mouse Monoclonal Antibody
Product Description google Mouse anti-Tubulin beta chain Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Alternative Names Tubb5; Tubulin beta-5 chain; TUBB; TUBB1; TUBB2A; TUBB2B; TUBB2C; TUBB3; TUBB4;
Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Species Reactivity Human, Rat
Immunogen Description beta-Tubulin from rat brain
Conjugate Unconjugated
Purity Description IgG
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Tubulin beta chain Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human Tubulin beta chain has a predicted length of 444 residues and MW of 50 kDa. A concentration of 0.5-1.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Tubulin beta chain
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Target Host Species Rat
Species Reactivity Human, Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG
Clone Name Tub-2
Conjugate Unconjugated
Immunogen Description beta-Tubulin from rat brain
Purity Description IgG
Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Tubb5; Tubulin beta-5 chain; TUBB; TUBB1; TUBB2A; TUBB2B; TUBB2C; TUBB3; TUBB4;
Uniprot Number P69897
Uniprot Number/Name P69897 (TBB5_RAT)
Scientific Background The tubulin family includes five distinct tubulins; alpha, beta, gamma, delta and epsilon. Microtubules consist principally of alpha- and beta-tubulin. Beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Beta tubulin immunoreactivity was detected in floated cryo-sections of the rat cerebral cortex with the M-1191-100 monoclonal antibody (0.2 µg/mL), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Note the intense labeling of the apical dendrites of pyramidal neurons. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Citations & References

Specific References Oh H. et al (2013) Uncovering a role for endocannabinoid signaling in autophagy in preimplantation mouse embryos. Mol Hum Reprod. 2013 Feb;19(2):93-101.