Product DescriptionMouse anti-Alpha-fetoprotein Monoclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s)IHC-Frozen
Application DetailsImmunohistochemistry (IHC). A concentration of 2.0-4.0 µg/mL is recommended to detect alpha fetoprotein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAlpha-fetoprotein
SpecificityThe specificity of this antibody has been confirmed by IHC against the antigen. Human;
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameAP-1
ConjugateUnconjugated
Immunogen DescriptionPurified human alpha-fetoprotein
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundAlpha-fetoprotein is a serum protein produced during fetal development and by hepatocytes. High levels of expression in adults is associated with hepatoma or teratoma.
Immunohistochemical detection of human alpha-fetoprotein in paraffin-embedded human liver cancer tissue using mouse monoclonal antibody to alpha-fetoprotein (1:200), catalogue number M-965-100 followed by incubation with goat anti-mouse IgG antibody.