Application DetailsWestern Blotting (WB). A concentration of 0.25-0.5 µg/mL is recommended for WB. Human Tubulin gamma-1 chain has a predicted length of 451 residues and MW of 51 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTubulin gamma-1 chain
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameIMD-20
ConjugateUnconjugated
Immunogen DescriptionSynthetic peptide (EEFATEGTDRKDVFFY) conjugated to KLH corresponding to the region of amino acids 38-53 of human gamma-Tubulin.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe tubulin family includes five distinct tubulins; alpha, beta, gamma, delta and epsilon. Gamma-tubulin has a role in the nucleation and polar orientation of microtubules and is found at the microtubule organizing centers such as the spindle poles or the centrosome. The antigen sequence is found in both human gamma-tubulin 1 and gamma-tubulin 2.