1800 605-5127
+61 (0)8 8352 7711
Calcium/calmodulin-dependent protein kinase 1, Mouse Monoclonal Antibody
As low as
US$417.00
Only %1 left
Catalog Number
M-810
- Product Name Calcium/calmodulin-dependent protein kinase 1, Mouse Monoclonal Antibody
- Product Description Mouse anti-Calcium/calmodulin-dependent protein kinase 1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
- Alternative Names Calcium/calmodulin-dependent protein kinase type 1; CaM-KI; CaM kinase I alpha; CAMKI-alpha; CAMK1
- Application(s) ELISA, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The specificity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged CaM Kinase 1 protein. No other species have been tested yet.
- Species Reactivity Human
- Immunogen Description Partial recombinant protein of human CaM kinase 1 (amino acids 271 to 370) with a GST tag.
- Conjugate Unconjugated
- Purity Description Protein G purified immunoglobulin
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Calcium/calmodulin-dependent protein kinase 1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
- Application(s) ELISA, WB
- Application Details This antibody is recommended for WB and both direct and sandwich ELISA. This antibody has a detection limit of 0.3ng/mL for recombinant GST tagged CaM kinase 1 when used in sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Calcium/calmodulin-dependent protein kinase 1
- Specificity The specificity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged CaM Kinase 1 protein. No other species have been tested yet.
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name 3G1
- Conjugate Unconjugated
- Immunogen Description Partial recombinant protein of human CaM kinase 1 (amino acids 271 to 370) with a GST tag.
- Purity Description Protein G purified immunoglobulin
- Format Lyophilized from PBS pH 7.2
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Calcium/calmodulin-dependent protein kinase type 1; CaM-KI; CaM kinase I alpha; CAMKI-alpha; CAMK1
- Uniprot Number Q14012
- Uniprot Number/Name Q14012 (KCC1A_HUMAN)
-
Scientific Background
CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
