Application DetailsThis antibody is recommended for WB, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 50 µg/mL for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeurogenin-1 (NGN-1)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name4A2
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human Neurogenin-1 (82-164) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Neurogenin-1 appears to mediate neuronal differentiation. It requires dimerisation with another basic-helix-loop-helix protein for efficient DNA binding. Its expression is restricted to the embryonic nervous system.
mmunofluorescent detection of Neurogenin-1 exression in HeLa cells. The anti-Neurogenin-1 primary antibody, catalogue number M-851-100, was used at a concentration of 50 µg/mL.
Western blot detection of Neurogenin-1 in HeLa S3 nuclear extract lysate.