Product DescriptionMouse anti-Nuclear receptor subfamily 2 group E member 1 (TLX) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, ELISA and Flow Cytometry (~2 µg per 10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNuclear receptor subfamily 2 group E member 1 (TLX)
SpecificitySpecificity has been confirmed by direct ELISA against the antigen. Human, mouse and rat. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name1C4
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human Nuclear receptor TLX [248-342] with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNuclear receptor subfamily 2 group E member 1; Protein tailless homolog; TII; hTII; NR2E1; TLX
Nuclear receptor TLX is an orphan receptor that binds DNA as a monomer and shares a target gene specificity that is unique among the nuclear receptor superfamily. It is thought to be required for brain development and also involved in the regulation of retinal development. It is a brain-specific protein that is present in all brain section tested with highest levels in the caudate nucleus and hippocampus.
Left: Western blot detection of Nuclear receptor TLX expression in mouse NIH/ Swiss embryo cell lysate. Right: Analysis of Nuclear Receptor TLX expression in rat C6 glioma cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL normal sheep IgG, Primary antibody: Mouse monoclonal antibody to human Nuclear Receptor TLX (1C4, cat # M-920-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
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