Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Chicken Tropomyosin alpha-1 (isoform #1) has a predicted length of 284 residues and a MW of 33 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTropomyosin alpha-1 chain
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rabbit; chicken;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTropomyosin-1; Alpha-tropomyosin; Beta-tropomyosin; TPM1; TPM2; TPM3; TPM4; C15orf13; TMSA;
Scientific BackgroundTropomyosins are dimeric coiled coil proteins associated with the actin filaments of myofibrils and stress fibres. In vertebrates, at least 4 known genes code for diverse Tropomyosin isoforms that are expressed in a tissue-specific manner.