SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. This antibody cross reacts with mouse. Other species have not been tested.
Species ReactivityHuman, Mouse
Immunogen DescriptionPartial recombinant protein of human Ephrin-A5 (aa 114 to 204) with a GST tag.
ConjugateUnconjugated
Purity DescriptionProtein G purified immunoglobulin
Product DescriptionMouse anti-Ephrin-A51 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetEphrin-A51
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. This antibody cross reacts with mouse. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name1F12
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant protein of human Ephrin-A5 (aa 114 to 204) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Ephrin-A5 is a member of the ephrin (EPH) gene family. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ephrin-A5 prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation.
Western blot detection of Ephrin-A5 in mouse NIH/Swiss embryo whole cell lysate using Mouse monoclonal antibody to human Ephrin-A5, catalogue number M-832-100.