Product DescriptionMouse anti-Semaphorin-6A Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, ELISA and Flow Cytometry. Use 1:100 fold dilution for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSemaphorin-6A
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human Semaphorin 6A (21-120) with a GST tag.
Purity DescriptionWhole serum
FormatLiquid. 50% glycerol
Storage InstructionsKeep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSemaphorin VIA; Sema VIA; Semaphorin-6A-1; SEMA6A-1; SEMA6A; KIAA1368; SEMAQ
The transmembrane semaphorin Semaphorin 6A is expressed in developing neural tissue and is required for proper development of the thalamocortical neuron projection. Semaphorin 6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. It was also suggested that Semaphorin 6A has a role in retrograde signaling.
Left: Western blot detection of GST tagged recombinant human Semaphorin 6A. Note the GST tag alone is 26 kDa. Right: Analysis of SEMA6A expression in human neuroblastoma SH-SY5Y cell line by Flow Cytometry.Cells were washed twice using PBS + 0.1% NaN3, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to SEMA6A (cat # Mo-952-100, 1:100 fold dilution for ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
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