Neurofilament light polypeptide (NF-L), Chicken Polyclonal Antibody

Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb (red), and co-stained with product R-1388-50, Neurofilament heavy polypeptide phosphorylated (pNF-H), Rabbit pAb, (green). The Neurofilament Light (NF-L) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast, the phosphorylated Neurofilament Heavy (NF-H) has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.

Analysis by western blot of NF-L expression in different tissue lysates probed with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb, (green, 1:20,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: cow spinal cord. Note NF-L proteins are known to have slightly different apparent SDS-PAGE molecular weights across species.

Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb, (green, 1:2,000), and co-stained with product M-377-100, NeuN/Fox3, Clone 1B7, Mouse mAb, (red, 1:5000). IHC Method: Following transcardial perfusion of rat with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The NF-L antibody stains perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while product M-377-100 stains the nuclei and proximal cytoplasm of neurons.