Neurofilament medium polypeptide (NF-M), Chicken Polyclonal Antibody

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Catalog Number
C-1393

    Product Info

  • Product Name Neurofilament medium polypeptide (NF-M), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Neurofilament medium polypeptide (NF-M), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
  • Alternative Names NF-M; NFM; Neurofilament medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm
  • Application(s) IF, ICC, IHC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology.
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Other Mammals (Predicted), Pig, Rat
  • Immunogen Description This antibody has been made against a recombinant fusion protein containing the extreme C-terminus of human NF-M (amino acids 708-877) expressed in and purified from E. coli.
  • Conjugate Unconjugated
  • Purity Description IgY Preparation
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Neurofilament medium polypeptide (NF-M), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
  • Related Products Neurofilament medium polypeptide (NF-M), Mouse Monoclonal Antibody (3H11)
    Neurofilament medium polypeptide (NF-M), Rabbit Polyclonal Antibody
  • Application(s) IF, ICC, IHC, WB
  • Application Details Western blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF) and Immunohistochemistry (IHC). A dilution of 1:2,000 - 1:5,000 is recommended for WB. A dilution of 1:2,000 is recommended for ICC/IF and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Neurofilament medium polypeptide (NF-M)
  • Specificity Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology.
  • Target Host Species Human
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Other Mammals (Predicted), Pig, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description This antibody has been made against a recombinant fusion protein containing the extreme C-terminus of human NF-M (amino acids 708-877) expressed in and purified from E. coli.
  • Purity Description IgY Preparation
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names NF-M; NFM; Neurofilament medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm
  • Uniprot Number P07197
  • Uniprot Number/Name P07197 (NFM_HUMAN)
  • Scientific Background Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. These cultures were stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red). The Neurofilament Medium (NF-M) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.


  • Image of rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red, 1:1,000) and co-stained with product M-1802-50, 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP), Mouse mAb (green, 1:500). The blue is DAPI staining of nuclear DNA. Method: Following transcardial perfusion of rat with 4% PFA, brain was post-fixed for 24 hours, cut to 45 uM, and free-floating section was stained. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.


    Analysis by western blot of NF-M expression in neuronal tissue and cell lysates with C-1393-50 (green, 1:2000) with strong bands at 145kDa (rodent NF-M molecules) and 160 kDa (human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties.) Lane 1:protein standard; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: NIH/3T3 cells; Lane 7: HEK293; Lane 8: HeLa; Lane 9: SH-SY5Y; Lane 10: C6 cells. NF-M is not expressed in HeLa and other cell lines tested.