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Sonic Hedgehog protein (SHH), Rabbit Polyclonal Antibody
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Catalog Number
R-1082
- Product Name Sonic Hedgehog protein (SHH), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Sonic Hedgehog protein (SHH) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names SHH; HHG-1;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
- Species Reactivity Human, Mouse, Rabbit, Rat
- Immunogen Description A synthetic peptide (RHPKKLTPLAYKQFIPN) corresponding to a region (34-50) from human Sonic Hedgehog protein (SHH). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Sonic Hedgehog protein (SHH) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Sonic Hedgehog protein (precursor) has a predicted length of 462 amino acids and MW of 50 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Sonic Hedgehog protein (SHH)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
- Target Host Species Human
- Species Reactivity Human, Mouse, Rabbit, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (RHPKKLTPLAYKQFIPN) corresponding to a region (34-50) from human Sonic Hedgehog protein (SHH). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names SHH; HHG-1;
- Uniprot Number Q15465
- Uniprot Number/Name Q15465 (SHH_HUMAN
- Scientific Background Sonic Hedgehog protein (SHH) is essential for a variety of patterning events during embryo development. SHH is expressed in fetal intestine, liver, lung, and kidney. The SHH precursor protein is autocatalytically cleaved into two products. The C-terminal portion is involved in the proteolysis of the precursor and cholesterol transferase activity. The N-terminal portion contains signalling activity.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
