Vimentin, Mouse Monoclonal Antibody

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Catalog Number
M-2121

    Product Info

  • Product Name Vimentin, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Vimentin, Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Alternative Names Vimentin, VIM
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Human. Species cross-reactivity includes rat.
  • Species Reactivity Human, Rat
  • Immunogen Description The antibody has been made against the full length recombinant human vimentin protein expressed in and purified from E Coli. The antibody binds to a region in the C-terminal “tail” region of vimentin included in the peptide SRISLPLPNFSSLNLRE.
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Vimentin, Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Related Products Vimentin, Chicken Polyclonal Antibody
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    Vimentin, Rabbit Polyclonal Antibody
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Vimentin
  • Specificity Human. Species cross-reactivity includes rat.
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1
  • Clone Name 2A52
  • Conjugate Unconjugated
  • Immunogen Description The antibody has been made against the full length recombinant human vimentin protein expressed in and purified from E Coli. The antibody binds to a region in the C-terminal “tail” region of vimentin included in the peptide SRISLPLPNFSSLNLRE.
  • Purity Description Protein G purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Vimentin, VIM
  • Uniprot Number P08670
  • Uniprot Number/Name P08670 (VIME_HUMAN)
  • Scientific Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Vimentins are Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.( Ref: uniprot.org)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Visualization of vimentin expression in rat cortical neuron-glial cell culture by Immunofluorescent analysis. Section was stained with mouse anti-Vimentin antibody, M-2121-100, (green, 1:5,000) and costained with rabbit polyclonal antibody to GFAP, R-1374-50, (red, 1:5,000). DAPI staining of nuclear DNA (blue). Vimentin is contained in fibroblastic, microglial and developing astrocytic cells which appear green. Maruting astrocytic cells appear red or yellow as they contain varying amounts of GFAP and vimentin.Right: Analysis by western blot of whole brain tissue lysates with M-2121-100 (green, 1:5,000) with band at approx. 50kDa. Lane 1: protein standard (red); Lane 2: HEK293; Lane 3: HeLa; Lane 4: SH-SY5Y; Lane 5: COS-1; Lane 6: C6; Lane 7: rat brain.

    Citations & References