Product DescriptionChicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsELISA and WB. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
TargetLeptin
SpecificityHuman Leptin
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionRecombinant human Leptin protein
Purity DescriptionAffinity-purified
FormatLiquid. PBS with 0.02% Sodium Azide
Storage InstructionsShort term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesLeptin; Obesity factor; Lep; Ob; Obese protein;
Scientific BackgroundLeptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene).
Western blot analysis of leptin in Human. Lane 1: Rabbit polyclonal antibody to human Leptin (R-1552-100) at 1:1,000 Lane 2: Chicken polyclonal antibody to human Leptin (C-1510-500) at 1:1,000