Tyrosine Kinase Receptor B (TrkB), Mouse Monoclonal Antibody

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Catalog Number
M-853

    Product Info

  • Product Name Tyrosine Kinase Receptor B (TrkB), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB; Trk-B; TrkB; NTRK2; TRKB
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Recombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, sandwich ELISA and Flow Cytometry. Use 2 μg/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Tyrosine Kinase Receptor B (TrkB)
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG2a, kappa
  • Clone Name 4D3-F10
  • Conjugate Unconjugated
  • Immunogen Description Recombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB; Trk-B; TrkB; NTRK2; TRKB
  • Uniprot Number Q16620
  • Uniprot Number/Name Q16620 (NTRK2_HUMAN)
  • Scientific Background
    Tyrosine kinase receptor type B (TrkB) is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). It is involved in the development and/or maintenance of the nervous system. It exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. It is a tran-membrane protein. There are 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. The different forms are differentially expressed in various cell types including neurons and microglia.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Western blot detection of Tyrosine kinase receptor type B (TrkB) expression in human osterogenic sarcoma cell lysate. Right: Analysis of TrkB expression in human prostate cancer DU145 cell line by Flow Cytometry. DU145 cells washed twice using PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to TrkB (cat # M-853-100, 2 μg per ~10^6 cells) for 30 minutes on ice, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

    Citations & References

  • General References Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-86.
    Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90.
    Nakajima K et al. Glia. 1998 Nov;24(3):272-89.