Product DescriptionMouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, sandwich ELISA and Flow Cytometry. Use 2 μg/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTyrosine Kinase Receptor B (TrkB)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG2a, kappa
Clone Name4D3-F10
ConjugateUnconjugated
Immunogen DescriptionRecombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Tyrosine kinase receptor type B (TrkB) is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). It is involved in the development and/or maintenance of the nervous system. It exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. It is a tran-membrane protein. There are 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. The different forms are differentially expressed in various cell types including neurons and microglia.
Left: Western blot detection of Tyrosine kinase receptor type B (TrkB) expression in human osterogenic sarcoma cell lysate. Right: Analysis of TrkB expression in human prostate cancer DU145 cell line by Flow Cytometry. DU145 cells washed twice using PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to TrkB (cat # M-853-100, 2 μg per ~10^6 cells) for 30 minutes on ice, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
General ReferencesMizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-86. Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-89.