Tyrosine Kinase Receptor C (TrkC), Mouse Monoclonal Antibody

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Catalog Number
M-1837

    Product Info

  • Product Name Tyrosine Kinase Receptor C (TrkC), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Tyrosine Kinase Receptor C (TrkC) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
  • Alternative Names GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
  • Application(s) FC, ICC
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Human TrkC ECD, cross reactivity to rodent species is expected.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Synthetic peptide immunogen, KEPFPESTDNFI, cooresponding to amino acids 397-408 of human TrkC ECD.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin from tissue culture supernatants.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Tyrosine Kinase Receptor C (TrkC) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
  • Related Products Tyrosine Kinase Receptor C (TrkC), Rabbit Polyclonal Antibody
  • Application(s) FC, ICC
  • Application Details Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Immunocytochemistry (1-2 µg/mL): Tested on fixed (4% formaldehyde) human cells. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Tyrosine Kinase Receptor C (TrkC)
  • Specificity Human TrkC ECD, cross reactivity to rodent species is expected.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG2b, kappa
  • Clone Name BS337
  • Conjugate Unconjugated
  • Immunogen Description Synthetic peptide immunogen, KEPFPESTDNFI, cooresponding to amino acids 397-408 of human TrkC ECD.
  • Sequence KEPFPESTDNFI
  • Purity Description Protein G purified immunoglobulin from tissue culture supernatants.
  • Format Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose and contains no preservatives.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
  • Uniprot Number Q16288
  • Uniprot Number/Name Q16288 (NTRK3_HUMAN)
  • Scientific Background Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • TrkC expression (red) in human (HeLa, SHSY5Y, MCF-7) and rat x mouse hybrid cell line 50B11 analysed by Flow Cytometry. Staining was performed on unfixed cells on ice. Blocking: 100 µg/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkB clone BS337 (10 µg/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

  • TrkC expression (red) in 50B11 mouse x rat hybrid cell line analysed by Flow Cytometry. Fixation: 0 ,2 and 4% formaldehyde in PBS, pH 7.2-7.6 on ice (10 minutes). Blocking: 100 µg/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkC clone BS337 (10 µg/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.


    Membrane TrkC expression (green) in human HeLa cells, analyzed by Immunocytochemistry. Fixed (4% formaldehyde) and blocked (10% normal horse serum) HeLa cells were incubated with TrkC antibody M-1837-100 (2 µg/mL, green) for 1 hour. Cells were then permeabilized, blocked (10% horse serum, 0.1% Triton X-100) and incubated with rabbit NF-H antibody R-1389-50 (1:500, red) for 1 hour. Primary antibody binding was visualized with secondary donkey anti-mouse-CF488A and donkey anti-rabbit-CF568 antibodies (2 µg/mL, 1 hour incubation). Cell nuclei were stained with DAPI (blue). Negative control staining was performed under identical conditions with isotype control mouse antibody and rabbit whole serum from an non-immunized animal. Confocal imaging settings were the same for antibody and control staining. Magnification: 60x. Specific staining is observed for TrkC in the cell membrane, while the NF-H antibody reveals intracellular filaments.


    3D confocal imaging of membrane TrkC expression (green) in human HeLa cell. TrkC-IR is concentrated in the elongated protrusion away from the cell body, while intracellular NF-H expression (red) is observed throughout the cell. Blue: DAPI nuclear stain.

    Citations & References