Application DetailsFlow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Immunocytochemistry (1-2 µg/mL): Tested on fixed (4% formaldehyde) human cells. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTyrosine Kinase Receptor C (TrkC)
SpecificityHuman TrkC ECD, cross reactivity to rodent species is expected.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG2b, kappa
Clone NameBS337
ConjugateUnconjugated
Immunogen DescriptionSynthetic peptide immunogen, KEPFPESTDNFI, cooresponding to amino acids 397-408 of human TrkC ECD.
SequenceKEPFPESTDNFI
Purity DescriptionProtein G purified immunoglobulin from tissue culture supernatants.
FormatLyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose and contains no preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
Scientific BackgroundReceptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
TrkC expression (red) in human (HeLa, SHSY5Y, MCF-7) and rat x mouse hybrid cell line 50B11 analysed by Flow Cytometry. Staining was performed on unfixed cells on ice. Blocking: 100 µg/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkB clone BS337 (10 µg/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
TrkC expression (red) in 50B11 mouse x rat hybrid cell line analysed by Flow Cytometry. Fixation: 0 ,2 and 4% formaldehyde in PBS, pH 7.2-7.6 on ice (10 minutes). Blocking: 100 µg/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkC clone BS337 (10 µg/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Membrane TrkC expression (green) in human HeLa cells, analyzed by Immunocytochemistry. Fixed (4% formaldehyde) and blocked (10% normal horse serum) HeLa cells were incubated with TrkC antibody M-1837-100 (2 µg/mL, green) for 1 hour. Cells were then permeabilized, blocked (10% horse serum, 0.1% Triton X-100) and incubated with rabbit NF-H antibody R-1389-50 (1:500, red) for 1 hour. Primary antibody binding was visualized with secondary donkey anti-mouse-CF488A and donkey anti-rabbit-CF568 antibodies (2 µg/mL, 1 hour incubation). Cell nuclei were stained with DAPI (blue). Negative control staining was performed under identical conditions with isotype control mouse antibody and rabbit whole serum from an non-immunized animal. Confocal imaging settings were the same for antibody and control staining. Magnification: 60x. Specific staining is observed for TrkC in the cell membrane, while the NF-H antibody reveals intracellular filaments.
3D confocal imaging of membrane TrkC expression (green) in human HeLa cell. TrkC-IR is concentrated in the elongated protrusion away from the cell body, while intracellular NF-H expression (red) is observed throughout the cell. Blue: DAPI nuclear stain.