Skip to the beginning of the images gallery

Tyrosine Kinase Receptor B (TrkB), Rabbit Polyclonal Antibody

As low as US$317.00

Only %1 left

Catalog Number

R-149

Product Info

Product Name Tyrosine Kinase Receptor B (TrkB), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2
Application(s) ELISA, IHC-Frozen
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with rat and mouse TrkB. Other species have not yet been tested.
Species Reactivity Mouse, Rat
Immunogen Description Extracellular domain of glycosylated mouse TrkB protein produced in CHO cells was used as the immunogen. As shown for similar antisera, it is anticipated that this antibody will block the TrkB receptor binding of corresponding neurotrophin ligand.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Application(s) ELISA, IHC-Frozen
Application Details IF (1:1000-1:3000), 1-site ELISA (1:10,000 dilution). A dilution of 1:1000 to 1:3000 o/n is recommended for IF. 4% PFA frozen sections tested. Not yet tested on paraffin embedded tissues. Use triton X-100 permeabilizaiton with frozen sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Tyrosine Kinase Receptor B (TrkB)
Specificity Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with rat and mouse TrkB. Other species have not yet been tested.
Target Host Species Mouse
Species Reactivity Mouse, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description Extracellular domain of glycosylated mouse TrkB protein produced in CHO cells was used as the immunogen. As shown for similar antisera, it is anticipated that this antibody will block the TrkB receptor binding of corresponding neurotrophin ligand.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2
Uniprot Number P15209
Uniprot Number/Name P15209 (NTRK2_MOUSE)
Scientific Background TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain. Mutations in the TrkB gene have been associated with obesity and mood disorders.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Immunohistochemical staining of Tyrosine Kinase Receptor B (TrkB) in rat trigeminal nerve (free floating cryostat section) using rabbit antibody (R-149-100) at a dilution of 1 in 3000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.
Immunohistochemical staining of Tyrosine Kinase Receptor B (TrkB) in rat dorsal horn (free floating cryostat section) using rabbit antibody (R-149-100) at a dilution of 1 in 3000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

Immunohistochemical staining of Tyrosine Kinase Receptor B (TrkB) in rat ventral horn (free floating cryostat section) using rabbit antibody (R-149-100) at a dilution of 1 in 3000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

Citations & References

Specific References Rudolph M (2020). Mesolimbic GluA1 AMPA Receptor Signaling in Dopaminergic Neurons Plays a Critical Role in the Induction of Cross-Sensitization to Psychostimulants in Response to Social Stress. PhD Thesis. Application: IHC (IF), frozen sections. Species: Rat.

Penzo MA et al. (2015). The paraventricular thalamus controls a central amygdala fear circuit. Nature. [Epub ahead of print]. Application: IHC (IF), 4% PFA, frozen sections. Species: Mouse.
General References Desmet CJ, Peeper DS (2006) Cell Mol Life Sci. 63(7-8) pp. 755-9
Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-8
Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90.
Nakajima K et al. Glia. 1998 Nov;24(3):272-8