Product DescriptionRabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsWB and ELISA. Suggested dilution of 1:1,000-1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
TargetLeptin
SpecificityMouse, Rat
Target Host SpeciesRat
Species ReactivityMouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionRecombinant rat Leptin protein
Purity DescriptionWhole serum
FormatLiquid. PBS with 0.02% Sodium Azide
Storage InstructionsShort term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesLeptin; Obesity factor; Lep; Ob; Obese protein;
Scientific BackgroundLeptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Western blot analysis of leptin in Rat. Lane 1: Rabbit polyclonal antibody to rat Leptin (R-1553-100) at 1:1,000 Lane 2: Chicken polyclonal antibody to rat Leptin (C-1515-500) at 1:1,000