Product DescriptionMouse anti-LIM homeobox protein 1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetLIM homeobox protein 1
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name3G3
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant protein of human LIM homeobox protein 1 (aa 1 to 101) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesLIM/homeobox protein Lhx1; Homeobox protein 1; hLim-1; LHX1; LIM-1; LIM1
LIM homeobox protein 1 is a potential transcription factor that may play a role in differentiation and development of neural and lymphoid cells. It contains 1 homeobox DNA-binding domain and 2 LIM zinc-binding domains. The latter have a negative regulatory function and disruption of the LIM domains results in an activated form. It localises to the nucleus and is expressed in the brain, thymus and tonsils. It is also expressed in samples from patients with chronic myeloid leukemia and in 58% of acute myeloid leukemia cell lines.