SpecificitySpecificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognise at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
Application DetailsWestern Blotting (WB), Immunochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:2,000. The elFC2 protein has a predicted length of 860 residues and the MW of the monomer is 94 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognise at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.
Target Host SpeciesMouse
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
SequenceC-KAVQVHQDTLRTMYFA
Purity DescriptionAffinity purified
FormatLyophilized from PBS
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesProtein argonaute-1; Protein argonaute-2; Protein argonaute-3; Argonaute1; Argonaute2; Argonaute3; mAgo1; mAgo2; Eukaryotic translation initiation factor 2C; eIF-2C 2; eIF-2C 1; eIF2C 2; Piwi/argonaute family protein meIF2C2 Protein slicer; Eif2c2; Ago1; Ago2;
Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in(B) mouse hippocampal area. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.
Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in (D) mouse cerebellar cortex. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.
Western Blot in mouse brain extracts using Rabbit polyclonal to mouse elF2c protein (R-1556-100). Figure A: Total mouse brain extract. Detection at 0.03 µg/mL. Protein loaded 20 µg. Figure B: Mouse cytosolic brain extract. Detection at 0.03 µg/mL. Protein loaded 20 µg, 10 µg and 5 µg.
Specific ReferencesHuang KL, Chadee AB, Chen CY, Zhang Y, Shyu AB. (2013)"Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate." RNA. 2013 Jan 22. [Epub ahead of print]. Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.