Adenosine receptor A2b (ADORA2B), Goat Polyclonal Antibody

Product Info

Product Name Adenosine receptor A2b (ADORA2B), Goat Polyclonal Antibody
Product Description google Goat anti-Adenosine receptor A2b (ADORA2B) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Alternative Names ADORA2B; adenosine A2b receptor; HGNC:264; ADORA2; netrin-1 receptor
Application(s) FC, ICC, IHC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Adenosine A2b Receptor from Human.
Species Reactivity Human
Immunogen Description A synthetic peptide consisting of amino acids, CQADVKSGNGQ
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Adenosine receptor A2b (ADORA2B) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Application(s) FC, ICC, IHC, Pep-ELISA
Application Details Peptide ELISA (1:64000), Immunohistochemistry (2-4 µg/mL), Immunofluorescence ( 10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Adenosine receptor A2b (ADORA2B)
Specificity Reacts with Adenosine A2b Receptor from Human.
Target Host Species Human
Species Reactivity Human
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, CQADVKSGNGQ
Sequence CQADVKSGNGQ
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names ADORA2B; adenosine A2b receptor; HGNC:264; ADORA2; netrin-1 receptor
Uniprot Number P29275
Uniprot Number/Name P29275 (AA2BR_HUMAN)
Scientific Background Adenosine receptor A2b is a receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (Uniprot).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
A. Flow cytometric analysis: paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Human Kidney staining: (2 µg/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Citations & References