Atypical chemokine receptor 1 (ACKR1), Goat Polyclonal Antibody

Product Info

Product Name Atypical chemokine receptor 1 (ACKR1), Goat Polyclonal Antibody
Product Description google Goat anti-Atypical chemokine receptor 1 (ACKR1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, FC.
Alternative Names FY; DARC; HGNC:4035; Duffy blood group; CCBP1; GPD; CTA-134P22.3; CD234; FY; OTTHUMP00000060081; Duffy blood group, chemokine receptor; Duffy blood group antigen; Glycoprotein D; Dfy; GpFy; WBCQ1; Fy glycoprotein; OTTHUMP00000035293; OTTHUMP00000035294
Application(s) FC, IHC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Duffy / FY / DARC from Human. This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Species Reactivity Human
Immunogen Description A synthetic peptide consisting of amino acids, HRAELSPSTENSSQLDFED-C
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Atypical chemokine receptor 1 (ACKR1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, FC.
Application(s) FC, IHC, WB, Pep-ELISA
Application Details Peptide ELISA (1:6000), Western Blot (0.3-1 µg/mL), Immunohistochemistry (3 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Atypical chemokine receptor 1 (ACKR1)
Specificity Reacts with Duffy / FY / DARC from Human. This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Target Host Species Human
Species Reactivity Human
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, HRAELSPSTENSSQLDFED-C
Sequence HRAELSPSTENSSQLDFED
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names FY; DARC; HGNC:4035; Duffy blood group; CCBP1; GPD; CTA-134P22.3; CD234; FY; OTTHUMP00000060081; Duffy blood group, chemokine receptor; Duffy blood group antigen; Glycoprotein D; Dfy; GpFy; WBCQ1; Fy glycoprotein; OTTHUMP00000035293; OTTHUMP00000035294
Uniprot Number Q5Y7A2
Uniprot Number/Name Q5Y7A2 (Q5Y7A2_HUMAN)
Scientific Background The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot: (0.3 µg/mL) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (0.4 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Human lung staining: (5 µg/mL) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining. B. Human Kidney staining: (3 µg/mL) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

Citations & References