B-cell linker protein (BLINK), Goat Polyclonal Antibody

Product Info

Product Name B-cell linker protein (BLINK), Goat Polyclonal Antibody
Product Description google Goat anti-B-cell linker protein (BLINK) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Alternative Names BLNK; SLP-65; B-cell linker; BASH; BLNK-s; Ly57; MGC111051; SLP65; B cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; OTTHUMP00000020167; Src homology 2 domain-containing leukocyte protein of 65 kDa; BLNK-S; LY57; OTTHUMP00000020168
Application(s) FC, ICC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with BLNK / SLP-65 from Human. This antibody is expected to recognize all reported isoforms.
Species Reactivity Bovine (Predicted), Dog (Predicted), Human
Immunogen Description A synthetic peptide consisting of amino acids, C-KDSTRLKYAVKVS
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-B-cell linker protein (BLINK) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Application(s) FC, ICC, WB, Pep-ELISA
Application Details ELISA (1:32000), Western Blot (0.3-1 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target B-cell linker protein (BLINK)
Specificity Reacts with BLNK / SLP-65 from Human. This antibody is expected to recognize all reported isoforms.
Target Host Species Human
Species Reactivity Bovine (Predicted), Dog (Predicted), Human
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-KDSTRLKYAVKVS
Sequence KDSTRLKYAVKVS
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names BLNK; SLP-65; B-cell linker; BASH; BLNK-s; Ly57; MGC111051; SLP65; B cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; OTTHUMP00000020167; Src homology 2 domain-containing leukocyte protein of 65 kDa; BLNK-S; LY57; OTTHUMP00000020168
Uniprot Number Q8WV28
Uniprot Number/Name Q8WV28 (BLNK_HUMAN)
Scientific Background Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation (Uniprot)
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot: (0.3 µg/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). C. Flow cytometry analysis: paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References