Goat anti-Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, ICC, FC.
Alternative NamesCAMK2A; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; CAMKA; KIAA0968; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; OTTHUMP00000165787; OTTHUMP00000165788; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase IIA; calcium/calmodulin-dependent protein kinase type II alpha chain
Application(s)FC, ICC, IHC, WB, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with CAMK2A from Human, Mouse, Rat. This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-PRTAQSEETRVWHR
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Goat anti-Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, ICC, FC.
Application(s)FC, ICC, IHC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:164000), Western Blot (0.03-0.3 µg/mL), Immunohistochemistry (3.75 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCalcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A)
SpecificityReacts with CAMK2A from Human, Mouse, Rat. This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-PRTAQSEETRVWHR
SequencePRTAQSEETRVWHR
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCAMK2A; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; CAMKA; KIAA0968; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; OTTHUMP00000165787; OTTHUMP00000165788; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase IIA; calcium/calmodulin-dependent protein kinase type II alpha chain
Scientific BackgroundCalcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378).
Analysis by Immunofluorescence of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Analysis by western blot of G-1898-100 (1μg/ml) staining of Human Cerebral Cortex (A) + peptide (B), (0.5μg/ml) Human Cerebellum (C) + peptide (D), and (0.1ug/ml) Mouse Brain (E) + peptide (F) and Rat Brain (G) + peptide (H) lysate, (35μg protein in RIPA buffer). Detected by chemiluminescence
Analysis by flow cytometry of paraformaldehyde fixed Neuro2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.