Carabin (TBC1D10C), Goat Polyclonal Antibody

Product Info

Product Name Carabin (TBC1D10C), Goat Polyclonal Antibody
Product Description google Goat anti-Carabin (TBC1D10C) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
Alternative Names TBC1D10C; carabin; TBC1 domain family, member 10C; FLJ00332; MGC46488; TBC1 domain family member 10C;
Application(s) FC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Carabin / TBC1D10C from Human, Mouse.
Species Reactivity Dog (Predicted), Human, Mouse, Pig (Predicted), Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, C-PRRKPQTRGKTFH
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Carabin (TBC1D10C) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
Application(s) FC, WB, Pep-ELISA
Application Details Peptide ELISA (1:128000), Western Blot (0.3-2 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Carabin (TBC1D10C)
Specificity Reacts with Carabin / TBC1D10C from Human, Mouse.
Target Host Species Human
Species Reactivity Dog (Predicted), Human, Mouse, Pig (Predicted), Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-PRRKPQTRGKTFH
Sequence PRRKPQTRGKTFH
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names TBC1D10C; carabin; TBC1 domain family, member 10C; FLJ00332; MGC46488; TBC1 domain family member 10C;
Uniprot Number Q8IV04
Uniprot Number/Name Q8IV04 (TB10C_HUMAN)
Scientific Background Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras (Uniprot).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Fig 1. Western Blot Analysis: staining of Jurkat (A), (0.3 µg/mL) PBM (B) and negative control A431 (C) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence. Fig 2. Western Blot Analysis: (2 µg/mL) staining of Human Spleen (A) and Mouse Thymus (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence. Fig 3. Flow cytometric analysis: of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References