Caspase recruitment domain-containing protein 16 (CARD16), Goat Polyclonal Antibody

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Catalog Number
G-1869

    Product Info

  • Product Name Caspase recruitment domain-containing protein 16 (CARD16), Goat Polyclonal Antibody
  • Product Description google Goat anti-Caspase recruitment domain-containing protein 16 (CARD16) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
  • Alternative Names CARD16; COP; CARD only protein; PSEUDO-ICE; COP1; caspase-1 dominant-negative inhibitor pseudo-ICE; pseudo interleukin-1beta converting enzyme; caspase recruitment domain family, member 16; Pseudo-IL1B-converting enzyme;
  • Application(s) FC, ICC, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with COP1 / PSEUDO-ICE (Isofom 2) from Human. This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).
  • Species Reactivity Human
  • Immunogen Description A synthetic peptide consisting of amino acids, C-ETLGLSAGPIPGN
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Caspase recruitment domain-containing protein 16 (CARD16) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
  • Application(s) FC, ICC, Pep-ELISA
  • Application Details ELISA (1:4000), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Caspase recruitment domain-containing protein 16 (CARD16)
  • Specificity Reacts with COP1 / PSEUDO-ICE (Isofom 2) from Human. This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-ETLGLSAGPIPGN
  • Sequence ETLGLSAGPIPGN
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CARD16; COP; CARD only protein; PSEUDO-ICE; COP1; caspase-1 dominant-negative inhibitor pseudo-ICE; pseudo interleukin-1beta converting enzyme; caspase recruitment domain family, member 16; Pseudo-IL1B-converting enzyme;
  • Uniprot Number Q5EG05
  • Uniprot Number/Name Q5EG05 (CAR16_HUMAN)
  • Scientific Background Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References