CCN family member 2 (CCN2), Goat Polyclonal Antibody

Product Info

Product Name CCN family member 2 (CCN2), Goat Polyclonal Antibody
Product Description google Goat anti-CCN family member 2 (CCN2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names CTGF; connective tissue growth factor; CCN2; HCS24; IGFBP8; MGC102839; NOV2; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; OTTHUMP00000017213; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with CTGF from Human. Immunizing peptide overlaps disulphide bond.
Species Reactivity Bovine, Dog, Human, Mouse, Pig, Rat
Immunogen Description A synthetic peptide consisting of amino acids, TLPVEFKCPDGE
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-CCN family member 2 (CCN2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:64,000), Immunocytochemistry (10 µg/mL),Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target CCN family member 2 (CCN2)
Specificity Reacts with CTGF from Human. Immunizing peptide overlaps disulphide bond.
Target Host Species Human
Species Reactivity Bovine, Dog, Human, Mouse, Pig, Rat
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, TLPVEFKCPDGE
Sequence TLPVEFKCPDGE
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names CTGF; connective tissue growth factor; CCN2; HCS24; IGFBP8; MGC102839; NOV2; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; OTTHUMP00000017213; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
Uniprot Number P29279
Uniprot Number/Name P29279 (CCN2_HUMAN)
Scientific Background Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane and extracellular staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody

Citations & References