Product DescriptiongoogleGoat anti-E3 ubiquitin-protein ligase Itchy homolog (ITCH) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Alternative NamesITCH; AIF4; itchy homolog E3 ubiquitin protein ligase (mouse) ; AIP4; NAPP1; dJ468O1.1 ; NFE2-associated polypeptide 1; atrophin-1 interacting protein 4; dJ468O1.1 (atrophin 1 interacting protein 4, AIP4); itchy homolog E3 ubiquitin protein ligase; ubiquitin protein ligase ITCH
Application(s)FC, ICC, WB, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with ITCH / AIF4 from Human.
Species ReactivityBovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Rat (Predicted)
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-EIKSHDLKPNGGN
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-E3 ubiquitin-protein ligase Itchy homolog (ITCH) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Application(s)FC, ICC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:6000), Western Blot (0.1-0.3 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species ReactivityBovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Rat (Predicted)
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-EIKSHDLKPNGGN
SequenceEIKSHDLKPNGGN
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesITCH; AIF4; itchy homolog E3 ubiquitin protein ligase (mouse) ; AIP4; NAPP1; dJ468O1.1 ; NFE2-associated polypeptide 1; atrophin-1 interacting protein 4; dJ468O1.1 (atrophin 1 interacting protein 4, AIP4); itchy homolog E3 ubiquitin protein ligase; ubiquitin protein ligase ITCH
Scientific BackgroundInvolved in the control of inflammatory signaling pathways. Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (Uniprot).
A. Western Blot: (0.1 µg/mL) staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Detected bychemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5%
A. Immunofluorescence analysis: paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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