Forkhead box protein C2 (FOXC2), Goat Polyclonal Antibody

Product Info

Product Name Forkhead box protein C2 (FOXC2), Goat Polyclonal Antibody
Product Description google Goat anti-Forkhead box protein C2 (FOXC2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
Alternative Names FOXC2; forkhead box C2 (MFH-1, mesenchyme forkhead 1); MFH1; MFH-1; FKHL14; MFH-1,mesenchyme forkhead 1; forkhead (Drosophila)-like 14; forkhead, Drosophila, homolog-like 14; forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1); LD; forkhead box C2; forkhead, Drosophila, homolog-like 14
Application(s) FC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with FOXC2 from Human.
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, RHAAPYSYDCTKY
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Forkhead box protein C2 (FOXC2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
Application(s) FC, WB, Pep-ELISA
Application Details Peptide ELISA (1:4000), Western Blot (2 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Forkhead box protein C2 (FOXC2)
Specificity Reacts with FOXC2 from Human.
Target Host Species Human
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, RHAAPYSYDCTKY
Sequence RHAAPYSYDCTKY
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names FOXC2; forkhead box C2 (MFH-1, mesenchyme forkhead 1); MFH1; MFH-1; FKHL14; MFH-1,mesenchyme forkhead 1; forkhead (Drosophila)-like 14; forkhead, Drosophila, homolog-like 14; forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1); LD; forkhead box C2; forkhead, Drosophila, homolog-like 14
Uniprot Number Q99958
Uniprot Number/Name Q99958 (FOXC2_HUMAN)
Scientific Background This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot: (2 µg/mL) of nuclear HEK293 (A) and (1 µg/mL) negative control Human Pancreas (B) lysate [RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence. B. Flow cytometry analysis: paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. C. Staining of Human Breast Cancer: (2 µg/mL) staining of paraffin embedded Human Breast Cancer. Steamed antigen retrieval with Tris/EDTA buffer Ph 9, HRP-staining.

Citations & References