Fragile X mental retardation syndrome-related protein 1 (FXR1), Goat Polyclonal Antibody

Product Info

Product Name Fragile X mental retardation syndrome-related protein 1 (FXR1), Goat Polyclonal Antibody
Product Description google Goat anti-Fragile X mental retardation syndrome-related protein 1 (FXR1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Alternative Names FXR1; fragile X mental retardation, autosomal homolog 1 ; fragile X mental retardation-related protein 1
Application(s) FC, ICC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with FXR1 from Human, Mouse. This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse, Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, C-RIEGDNENKLPRED
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Fragile X mental retardation syndrome-related protein 1 (FXR1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Application(s) FC, ICC, WB, Pep-ELISA
Application Details Peptide ELISA (1:128000), Western Blot (1-3 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Fragile X mental retardation syndrome-related protein 1 (FXR1)
Specificity Reacts with FXR1 from Human, Mouse. This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
Target Host Species Human
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse, Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-RIEGDNENKLPRED
Sequence RIEGDNENKLPRED
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names FXR1; fragile X mental retardation, autosomal homolog 1 ; fragile X mental retardation-related protein 1
Uniprot Number P51114
Uniprot Number/Name P51114 (FXR1_HUMAN)
Scientific Background NA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs (By similarity) (Uniprot)
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot: (1 µg/mL) staining of NIH-3T3 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
A. Immunofluorescence analysis: paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometric analysis: paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References