Forkhead box protein P3 (FOXP3), Goat Polyclonal Antibody

Product Info

Product Name Forkhead box protein P3 (FOXP3), Goat Polyclonal Antibody
Product Description google Goat anti-Forkhead box protein P3 (FOXP3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
Alternative Names FOXP3; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; SCURFIN; scurfin; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
Application(s) FC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with FOXP3 from Human.
Species Reactivity Bovine, Human
Immunogen Description A synthetic peptide consisting of amino acids, CSNPTPGP
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Forkhead box protein P3 (FOXP3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
Application(s) FC, Pep-ELISA
Application Details ELISA (1:8000), Western Blot (1-3 µg/mL), Immunofluorescence ( 2-4 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Forkhead box protein P3 (FOXP3)
Specificity Reacts with FOXP3 from Human.
Target Host Species Human
Species Reactivity Bovine, Human
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, CSNPTPGP
Sequence SQRPSRCSNPTPGP
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names FOXP3; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; SCURFIN; scurfin; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
Uniprot Number Q9BZS1
Uniprot Number/Name Q9BZS1 (FOXP3_HUMAN)
Scientific Background Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Flow cytometry analysis: paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Western Blot: (1 µg/mL) staining of Human Muscle (A), (2 µg/mL) MOLT4 (B) and (1 µg/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. C. Human Tonsil: (1 µg/mL) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.
Human Blood Cells: staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).

Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).

Citations & References