Product NameIntegral membrane protein 2B (ITM2B), Goat Polyclonal Antibody
Product DescriptiongoogleGoat anti-Integral membrane protein 2B (ITM2B) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, ICC, FC.
Alternative NamesITM2B; integral membrane protein 2B; ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD; BRICHOS domain containing 2B; Immature BRI2; OTTHUMP00000018394; Transmembrane protein BRI;
Application(s)FC, ICC, IHC, WB, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with ITM2B from Human.
Species ReactivityBovine, Human, Mouse, Rat
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-HDKETYKLQRRETIK
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Integral membrane protein 2B (ITM2B) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, ICC, FC.
Application(s)FC, ICC, IHC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:8000), Western Blot (0.1-0.3 µg/mL), Immunohistochemistry (2.5 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetIntegral membrane protein 2B (ITM2B)
SpecificityReacts with ITM2B from Human.
Target Host SpeciesHuman
Species ReactivityBovine, Human, Mouse, Rat
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-HDKETYKLQRRETIK
SequenceHDKETYKLQRRETIK
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesITM2B; integral membrane protein 2B; ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD; BRICHOS domain containing 2B; Immature BRI2; OTTHUMP00000018394; Transmembrane protein BRI;
Scientific BackgroundPlays a regulatory role in the processing of the amyloid-beta A4 precursor protein (APP) and acts as an inhibitor of the amyloid-beta peptide aggregation and fibrils deposition. Plays a role in the induction of neurite outgrowth. Functions as a protease inhibitor by blocking access of secretases to APP cleavage sites.
A. Western Blot: (0.3 µg/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by Chemiluminescence B. Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Human Small Intestine Staining: (2.5 µg/mL) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
A. Immunofluorescence analysis: of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
1800 605-5127
+61 (0)8 8352 7711
