Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4), Goat Polyclonal Antibody

Product Info

Product Name Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4), Goat Polyclonal Antibody
Product Description google Goat anti-Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC, IHC.
Alternative Names KCNN4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; IK1; IKCA1; KCA4; KCa3.1; SK4; hIKCa1; hKCa4; hSK4; intermediate conductance calcium-activated potassium channel protein 1; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel
Application(s) FC, ICC, IHC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with KCNN4 / KCa3.1 from Human, Mouse.
Species Reactivity Dog, Human, Mouse, Rat
Immunogen Description A synthetic peptide consisting of amino acids, C-ERQAVNATGHLSD
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC, IHC.
Application(s) FC, ICC, IHC, WB, Pep-ELISA
Application Details Peptide ELISA (1:6,000), Western Blot (0.1-0.3 µg/mL), Immunohistochemistry (3.75 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4)
Specificity Reacts with KCNN4 / KCa3.1 from Human, Mouse.
Target Host Species Human
Species Reactivity Dog, Human, Mouse, Rat
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-ERQAVNATGHLSD
Sequence ERQAVNATGHLSD
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names KCNN4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; IK1; IKCA1; KCA4; KCa3.1; SK4; hIKCa1; hKCa4; hSK4; intermediate conductance calcium-activated potassium channel protein 1; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel
Uniprot Number O15554
Uniprot Number/Name O15554 (KCNN4_HUMAN)
Scientific Background Forms a voltage-independent potassium channel that is activated by intracellular calcium (PubMed:26148990). Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed:17157250, PubMed:18796614). Plays a role in the late stages of EGF-induced macropinocytosis (PubMed:24591580).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Western Blot:(0.1 µg/mL) staining of Human Cerebellum (A) + Peptide (B), NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
A. Human Kidney Staining: (3.75 µg/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. B. Human Liver Staining: (3.75 µg/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

A. Flow cytometric analysis: of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight at 4°C (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Human Adrenal Gland Staining: (3.75 µg/mL) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Citations & References