Lymphocyte antigen 6E (Ly-6E), Goat Polyclonal Antibody

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Catalog Number
G-1902

    Product Info

  • Product Name Lymphocyte antigen 6E (Ly-6E), Goat Polyclonal Antibody
  • Product Description google Goat anti-Lymphocyte antigen 6E (Ly-6E) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
  • Alternative Names LY6E; lymphocyte antigen 6 complex, locus E; RIG-E; RIGE; SCA-2; SCA2; TSA-1; retinoic acid induced gene E; Stem cell antigen 2; Thymic shared antigen 1;
  • Application(s) FC, ICC, IHC, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with LY6E (mouse) from Mouse. Reported variants represent identical protein: NP_002337.1, NP_001120685.1.
  • Species Reactivity Human, Mouse
  • Immunogen Description A synthetic peptide consisting of amino acids, SCQEKDHYCIT
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Lymphocyte antigen 6E (Ly-6E) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
  • Application(s) FC, ICC, IHC, Pep-ELISA
  • Application Details Peptide ELISA (1:16000), Western Blot (1 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Lymphocyte antigen 6E (Ly-6E)
  • Specificity Reacts with LY6E (mouse) from Mouse. Reported variants represent identical protein: NP_002337.1, NP_001120685.1.
  • Target Host Species Mouse
  • Species Reactivity Human, Mouse
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, SCQEKDHYCIT
  • Sequence SCQEKDHYCIT
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names LY6E; lymphocyte antigen 6 complex, locus E; RIG-E; RIGE; SCA-2; SCA2; TSA-1; retinoic acid induced gene E; Stem cell antigen 2; Thymic shared antigen 1;
  • Uniprot Number Q64253
  • Uniprot Number/Name Q64253 (LY6E_MOUSE)
  • Scientific Background Involved in T-cell development. Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-3:beta-4-containing nAChRs maximum response.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Immunofluorescence analysis: of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

  • Flow cytometric analysis: of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References