Left: Western blot detection of Nuclear receptor TLX expression in mouse NIH/ Swiss embryo cell lysate. Right: Analysis of Nuclear Receptor TLX expression in rat C6 glioma cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL normal sheep IgG, Primary antibody: Mouse monoclonal antibody to human Nuclear Receptor TLX (1C4, cat # M-920-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.