A: Specific staining of rat p75NTR expressed in rat C6 glioma cell line by Flow Cytometry using cat # M-006-100. Blocking: 200 µg/mL normal sheep IgG, Primary antibody: MC192 (2 μg per ~10^6 cells), 30 minutes in ice, Secondary antibody: Goat anti-mouse PE (1:100), 20 minutes in dark at room temperature. Negative control: Non-specific Control IgG, clone X63 (cat # M-1249-200, black dashed). Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG) using Mouse monoclonal antibody to rat p75NTR [MC192]. Merged pictures of cultured rat DRG triple-stained for p75 NTR (red colour), beta-Tubulin (green colour) and nuclei using DAPI (violet colour). C: Western blot analysis of p75NTR expression in PC12 cells (RIPA lysate); SDS-PAGE: Denaturing (10 min @ 70°C), non-reducing; Electrophoresis: 4-20% Bis-Tris; Transfer: Tris-Glycine buffer, nitrocellulose membrane (0.45 µm); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: 5 and 10 µg/mL MC192 in blocking buffer, overnight incubation; Secondary antibody: Anti-mouse-HRP (1:6000); Detection: Chemiluminiscence; Predicted MW of rat p75 based on amino acid sequence: 43 kDa; Observed MW due to post-translational modifications: 60 kDa. D: Immunohistochemical staining of p75NTR in rat motor neurons on lesioned sciatic nerve.