N-acetylaspartate synthetase (NAA synthetase), Goat Polyclonal Antibody

Product Info

Product Name N-acetylaspartate synthetase (NAA synthetase), Goat Polyclonal Antibody
Product Description google Goat anti-N-acetylaspartate synthetase (NAA synthetase) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Alternative Names Nat8l; N-acetyltransferase 8-like; 1110038O08Rik; Shati; N-acetylaspartate synthetase; N-acetyltransferase 8-like protein; NAA synthetase; protein Shati, Camello-like protein 3;
Application(s) FC, ICC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Nat8l (mouse aa211-223) from Human, Mouse.
Species Reactivity Bovine, Dog, Human, Mouse, Rat
Immunogen Description A synthetic peptide consisting of amino acids, C-SVDSRFRGKGIAK
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-N-acetylaspartate synthetase (NAA synthetase) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Application(s) FC, ICC, WB, Pep-ELISA
Application Details Peptide ELISA (1:128000), Western Blot (0.1-0.3 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target N-acetylaspartate synthetase (NAA synthetase)
Specificity Reacts with Nat8l (mouse aa211-223) from Human, Mouse.
Target Host Species Human
Species Reactivity Bovine, Dog, Human, Mouse, Rat
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-SVDSRFRGKGIAK
Sequence SVDSRFRGKGIAK
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Nat8l; N-acetyltransferase 8-like; 1110038O08Rik; Shati; N-acetylaspartate synthetase; N-acetyltransferase 8-like protein; NAA synthetase; protein Shati, Camello-like protein 3;
Uniprot Number Q8N9F0
Uniprot Number/Name Q8N9F0 (NAT8L_HUMAN)
Scientific Background Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot: (0.1 µg/mL) staining of Brain of fetal Mouse (A) + peptide (B), Mouse (C) + peptide (D), Human (E) + peptide (F) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis: of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Citations & References