Nuclear factor interleukin-3-regulated protein (NFIL3), Goat Polyclonal Antibody

As low as US$397.00
Only %1 left
Catalog Number
G-1868

    Product Info

  • Product Name Nuclear factor interleukin-3-regulated protein (NFIL3), Goat Polyclonal Antibody
  • Product Description google Goat anti-Nuclear factor interleukin-3-regulated protein (NFIL3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
  • Alternative Names NFIL3; nuclear factor, interleukin 3 regulated; E4BP4; IL3BP1; NFIL3A; NF-IL3A; interleukin-3 promoter transcriptional activator; OTTHUMP00000021633; Transcriptional activator NF-IL3A;
  • Application(s) FC, WB, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with NFIL3 from Human.
  • Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
  • Immunogen Description A synthetic peptide consisting of amino acids, C-RLIATQPISASDSG
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Nuclear factor interleukin-3-regulated protein (NFIL3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
  • Application(s) FC, WB, Pep-ELISA
  • Application Details ELISA (1:2000), Western Blotting (1-3 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Nuclear factor interleukin-3-regulated protein (NFIL3)
  • Specificity Reacts with NFIL3 from Human.
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-RLIATQPISASDSG
  • Sequence RLIATQPISASDSG
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names NFIL3; nuclear factor, interleukin 3 regulated; E4BP4; IL3BP1; NFIL3A; NF-IL3A; interleukin-3 promoter transcriptional activator; OTTHUMP00000021633; Transcriptional activator NF-IL3A;
  • Uniprot Number Q16649
  • Uniprot Number/Name Q16649 (NFIL3_HUMAN)
  • Scientific Background NFIL-3 is a protein coding gene that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Western Blot: (1 µg/mL) staining of Jurkat nuclear cell lysate (RIPA buffer, 20 ug total protein per lane). Detected using chemiluminescence B. Flow cytometry analysis: paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References