SpecificityReacts with Oxytocin Receptor from Human.
Species ReactivityHuman
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-LRTTRQKHSRL
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application DetailsPeptide ELISA (1:64000), Western Blot (2 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetOxytocin receptor (OT-R)
SpecificityReacts with Oxytocin Receptor from Human.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-LRTTRQKHSRL
SequenceLRTTRQKHSRL
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesOXTR; oxytocin receptor; OT-R; OTR
Scientific BackgroundReceptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system (Uniprot).
Flow cytometry analysis: of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. G-1885-100 (8 μg/mL) staining of paraffin embedded Human Ovary. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. B. Negative Control Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody