Parvalbumin, Goat Polyclonal Antibody

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Catalog Number
G-1877

    Product Info

  • Product Name Parvalbumin, Goat Polyclonal Antibody
  • Product Description google Goat anti-Parvalbumin Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC
  • Alternative Names parvalbumin; HGNC:9704; PVALB; D22S749; parvalbumin alpha; MGC116759
  • Application(s) FC, IHC, WB, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with Parvalbumin from Human, Rat. Reported variants represent identical protein: NP_002845.1, NP_001302461.1.
  • Species Reactivity Human, Rat
  • Immunogen Description A synthetic peptide consisting of amino acids, C-GVDEFSTLVAES
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Parvalbumin Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC
  • Application(s) FC, IHC, WB, Pep-ELISA
  • Application Details Peptide ELISA (1:32000), Western Blot (0.1-0.3 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Parvalbumin
  • Specificity Reacts with Parvalbumin from Human, Rat. Reported variants represent identical protein: NP_002845.1, NP_001302461.1.
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-GVDEFSTLVAES
  • Sequence GVDEFSTLVAES
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names parvalbumin; HGNC:9704; PVALB; D22S749; parvalbumin alpha; MGC116759
  • Uniprot Number P20472
  • Uniprot Number/Name P20472 (PRVA_HUMAN)
  • Scientific Background In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions (Uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Western Blot: (0.01 µg/mL) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3 µg/mL) Rat Brain (C) lysate + peptide (D) (35 µg/ protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry: analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1h (10 µg/mL) followed by Alexa Fluor 488 antibody (1 µg/mL). IgG control. Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References