Phosphatidate phosphatase LPIN2, Goat Polyclonal Antibody

Product Info

Product Name Phosphatidate phosphatase LPIN2, Goat Polyclonal Antibody
Product Description google Goat anti-Phosphatidate phosphatase LPIN2 Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names LPIN2; KIAA0249; lipin 2;
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Lipin 2 from Human.
Species Reactivity Human, Mouse (Predicted), Pig (Predicted), Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, CYWRDPIPEVDLDDLS
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Phosphatidate phosphatase LPIN2 Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Immunofluoresence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Phosphatidate phosphatase LPIN2
Specificity Reacts with Lipin 2 from Human.
Target Host Species Human
Species Reactivity Human, Mouse (Predicted), Pig (Predicted), Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, CYWRDPIPEVDLDDLS
Sequence CYWRDPIPEVDLDDLS
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names LPIN2; KIAA0249; lipin 2;
Uniprot Number Q92539
Uniprot Number/Name Q92539 (LPIN2_HUMAN)
Scientific Background Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism (Uniprot).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Flow cytometric analysis: paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Immunofluorescence analysis: paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Citations & References