Platelet-derived growth factor subunit B (PDGFB), Goat Polyclonal Antibody

Product Info

Product Name Platelet-derived growth factor subunit B (PDGFB), Goat Polyclonal Antibody
Product Description google Goat anti-Platelet-derived growth factor subunit B (PDGFB) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names PDGFB; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); FLJ12858; PDGF2; SIS; SSV; c-sis; PDGF, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); becaplermin; oncogene SIS; platelet-derived growth factor 2; platelet-derived growth factor beta; platelet-derived growth factor, B chain; v-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with PDGFB precursor from Human. This antibody is expected to recognize isoform 1 and 2 (NP_002599.1; NP_148937.1).
Species Reactivity Bovine, Dog, Human, Mouse, Rat, Sheep
Immunogen Description A synthetic peptide consisting of amino acids, C-KHRKFKHTHDKT
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Platelet-derived growth factor subunit B (PDGFB) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:128,000), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Platelet-derived growth factor subunit B (PDGFB)
Specificity Reacts with PDGFB precursor from Human. This antibody is expected to recognize isoform 1 and 2 (NP_002599.1; NP_148937.1).
Target Host Species Human
Species Reactivity Bovine, Dog, Human, Mouse, Rat, Sheep
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-KHRKFKHTHDKT
Sequence KHRKFKHTHDKT
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names PDGFB; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); FLJ12858; PDGF2; SIS; SSV; c-sis; PDGF, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); becaplermin; oncogene SIS; platelet-derived growth factor 2; platelet-derived growth factor beta; platelet-derived growth factor, B chain; v-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
Uniprot Number P01127
Uniprot Number/Name P01127 (PDGFB_HUMAN)
Scientific Background Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References