Plexin-D1 (PLXND1), Goat Polyclonal Antibody

Product Info

Product Name Plexin-D1 (PLXND1), Goat Polyclonal Antibody
Product Description google Goat anti-Plexin-D1 (PLXND1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC.
Alternative Names PLXND1; PLEXD1; KIAA0620; plexin D1; MGC75353
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Plexin D1 from Human.
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Pig (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, C-LAEPKKSHRQSH
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Plexin-D1 (PLXND1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:128000), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Plexin-D1 (PLXND1)
Specificity Reacts with Plexin D1 from Human.
Target Host Species Human
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Pig (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-LAEPKKSHRQSH
Sequence LAEPKKSHRQSH
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names PLXND1; PLEXD1; KIAA0620; plexin D1; MGC75353
Uniprot Number Q9Y4D7
Uniprot Number/Name Q9Y4D7 (PLXD1_HUMAN)
Scientific Background Plexin-D1 (PLXD1) plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (By similarity). Mediates anti-angiogenic signaling in response to SEMA3E (Uniprot).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Flow cytometry analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. G-1876-100 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
B. G-1876-100 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Citations & References