Product DescriptiongoogleGoat anti-Protein phosphatase 1 regulatory subunit 15A (PPP1R15A) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, FC.
Alternative NamesPPP1R15A; GADD34; protein phosphatase 1, regulatory (inhibitor) subunit 15A; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A
Application(s)FC, IHC, WB, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with PPP1R15A / GADD34 from Human.
Species ReactivityHuman
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-AAALDLSGRRG
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Protein phosphatase 1 regulatory subunit 15A (PPP1R15A) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, FC.
Application(s)FC, IHC, WB, Pep-ELISA
Application DetailsELISA (1:28000), Western Blotting (0.1-1 µg/mL), Immunocytochemistry (2-4 µg/mL), Immunofluorence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityReacts with PPP1R15A / GADD34 from Human.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-AAALDLSGRRG
SequenceAAALDLSGRRG
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesPPP1R15A; GADD34; protein phosphatase 1, regulatory (inhibitor) subunit 15A; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A
Scientific BackgroundActs as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters (Uniprot).
A. Flow cytometry analysis: paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (0.4 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Immunofluorescence analysis: paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL). C. Western Blot: (0.3 µg/mL) staining of HepG2 (A) and negative control KLY (B) lysate (35 ug protein in RIPA buffer) Detected by chemiluminescence
A. Human Liver Cell Staining: (2 µg/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. B. Immunofluorescence analysis: paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).
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