Receptor tyrosine-protein kinase erbB-3 (ERBB3), Goat Polyclonal Antibody

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Catalog Number
G-1879

    Product Info

  • Product Name Receptor tyrosine-protein kinase erbB-3 (ERBB3), Goat Polyclonal Antibody
  • Product Description google Goat anti-Receptor tyrosine-protein kinase erbB-3 (ERBB3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC, IHC.
  • Alternative Names ERBB3; HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); HGNC:3431; ErbB-3; MDA-BF-1; c-erbB-3; c-erbB3; erbB3-S; p180-ErbB3; p45-sErbB3; p85-sErbB3 ; erbB-3; LCCS2; MGC88033; receptor tyrosine-protein kinase erbB-3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
  • Application(s) FC, ICC, IHC, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with ERBB3 / HER3 from Human. This antibody is expected to recognise isoform 1 (NP_001973.2) only.
  • Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Pig (Predicted), Rat (Predicted)
  • Immunogen Description A synthetic peptide consisting of amino acids, C-HSRLFPKANAQRT
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Receptor tyrosine-protein kinase erbB-3 (ERBB3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC, IHC.
  • Application(s) FC, ICC, IHC, Pep-ELISA
  • Application Details Peptide ELISA (1:32000), Immunohistochemistry (5 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Receptor tyrosine-protein kinase erbB-3 (ERBB3)
  • Specificity Reacts with ERBB3 / HER3 from Human. This antibody is expected to recognise isoform 1 (NP_001973.2) only.
  • Target Host Species Human
  • Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Pig (Predicted), Rat (Predicted)
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-HSRLFPKANAQRT
  • Sequence HSRLFPKANAQRT
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names ERBB3; HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); HGNC:3431; ErbB-3; MDA-BF-1; c-erbB-3; c-erbB3; erbB3-S; p180-ErbB3; p45-sErbB3; p85-sErbB3 ; erbB-3; LCCS2; MGC88033; receptor tyrosine-protein kinase erbB-3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
  • Uniprot Number P21860
  • Uniprot Number/Name P21860 (ERBB3_HUMAN)
  • Scientific Background Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation (Uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Immunofluorescence analysis: of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing plasma membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. C. Human Lung Staining: (5 µg/mL) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

    Citations & References