Roundabout homolog 3 (ROBO3), Goat Polyclonal Antibody

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Catalog Number
G-1884

    Product Info

  • Product Name Roundabout homolog 3 (ROBO3), Goat Polyclonal Antibody
  • Product Description google Goat anti-Roundabout homolog 3 (ROBO3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
  • Alternative Names ROBO3; roundabout, axon guidance receptor, homolog 3 (Drosophila) ; HGNC:13433; FLJ21044; HGPPS; RBIG1; RIG1 ; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3; Roundabout-like protein 3;
  • Application(s) FC, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with ROBO3 (internal) from Human.
  • Species Reactivity Dog (Predicted), Human
  • Immunogen Description A synthetic peptide consisting of amino acids, C-RKDGARLKEEEGR
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Roundabout homolog 3 (ROBO3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
  • Application(s) FC, Pep-ELISA
  • Application Details Peptide ELISA (1:64000),Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Roundabout homolog 3 (ROBO3)
  • Specificity Reacts with ROBO3 (internal) from Human.
  • Target Host Species Human
  • Species Reactivity Dog (Predicted), Human
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-RKDGARLKEEEGR
  • Sequence RKDGARLKEEEGR
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names ROBO3; roundabout, axon guidance receptor, homolog 3 (Drosophila) ; HGNC:13433; FLJ21044; HGPPS; RBIG1; RIG1 ; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3; Roundabout-like protein 3;
  • Uniprot Number Q96MS0
  • Uniprot Number/Name Q96MS0 (ROBO3_HUMAN)
  • Scientific Background Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube (By similarity). In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing Uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Flow cytometry analysis: of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References