SpecificityReacts with ROBO3 (internal) from Human.
Species ReactivityDog (Predicted), Human
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-RKDGARLKEEEGR
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Roundabout homolog 3 (ROBO3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
Application(s)FC, Pep-ELISA
Application DetailsPeptide ELISA (1:64000),Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetRoundabout homolog 3 (ROBO3)
SpecificityReacts with ROBO3 (internal) from Human.
Target Host SpeciesHuman
Species ReactivityDog (Predicted), Human
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-RKDGARLKEEEGR
SequenceRKDGARLKEEEGR
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThought to be involved during neural development in axonal navigation at the ventral midline of the neural tube (By similarity). In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing Uniprot).